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MGMT Protein(M354-30H)

MGMT Protein(M354-30H)

  • $215.00


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Description :Recombinant full length human MGMT was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :HIS tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :BC000824

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~25 kDa

Gene Aliases :(None)

Scientific Background :MGMT or O-6-methylguanine-DNA methyltransferase is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. The methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness of the tumors to alkylating agents (1). The clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide (2). Methylation of the MGMT promoter in tumors is associated with longer survival in glioblastoma patients.

References :
1. Esteller, M. et.al: Inactivation of the DNA-repair gene MGMT and the clinical response of gliomas to alkylating agents. New Eng. J. Med. 343: 1350-1354, 2000.

2. Hegi, M. E. et.al: Clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide. Clin. Cancer Res. 10: 1871-1874, 2004.

Product Sheets (By Lot #) :

F660-2.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle