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MBP Protein(M42-51N)

MBP Protein(M42-51N)

  • $50.00


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Description :Native Swine MBP was extracted under acidic conditions and further purified by cation chromatography from pig brain (1). This product is routinely evaluated using active MAP Kinase 3/ERK1.

Species :Swine

Tag :

Expression System:

Sequence :

Genbank Number :BC055439

Species Comparison :Sample Protein comparison chart. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –20oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~21.5 kDa

Scientific Background :MBP exists as four major forms in the pig CNS with apparent molecular weights of 21.5K, 20.2K, 18.5K, and 17.3K (2). Native pig MBP is extracted under acidic conditions and further purified by cation chromatography. MBP is an efficient substrate for numerous protein kinases including ERK/MAP Kinase family, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Protein Kinase C, and Phosphorylase Kinase (3, 4).

References :
1. Chevalier D, et al. Purification of myelin basic protein from bovine brain. Protein Expr Purif. 18(2):229-34, 2000.

2. Sheng HZ, et al. Developmental study of myelin basic protein variants in various regions of pig nervous system. J Neurochem. 1989 Mar;52(3):736-40.

3. Sanghera J. et al. Identification of the sites in myelin basic protein that are phosphorylated by meiosis-activated protein kinase p44mpk. FEBS Lett. 1990 Oct 29;273(1-2):223-6.

4. Martenson, et al., Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein. J. Biol. Chem. 258: 930, 1983.

Product Sheets (By Lot #) :

L085-4.pdf

R195-4.pdf

J617-6.pdf

O941-4.pdf

O965-4.pdf

T1026-6.pdf

T1026-5.pdf

O1025-5.pdf

K1852-3.pdf

Y1055-4.pdf

P1590-9.pdf

V2514-10.pdf

V2550-9.pdf

 

E3237-5.pdf

N3579-5.pdf

Research Areas :Neurobiology, ERK/MAPK Pathway, Neurobiology, ERK/MAPK Pathway