Anti-phospho-MET (Tyr1349 Tyr1356)(M52-65R)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human c-MET phosphorylated at Tyrosine 1349 and 1356
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse and Rat
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to residues surrounding Tyr1349 and Tyr1356 of human c-MET protein.
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment.
Sample Data :Western Blot using Anti-phospho-MET (Tyr1349 Tyr1356) antibody shows detection of phosphorylated c-Met in Human mammary B5/589 epithelial cells serum-deprived and treated with or without HGF.
Scientific Background :c-MET is a receptor tyrosine kinase that binds hepatocyte growth factor (also known as scatter factor, HGF/SF). Stimulation by HGF causes c-MET to autophosphorylate at multiple tyrosines, including Y1234 and Y1235. Activated c-MET binds to various substrates, such as GAB1, GRB2, phosphatidylinositol 3-kinase (PI3K) and others. GAB1 is scaffold protein, which enables c-MET to elicit downstream signaling cascades, which include the well-characterized ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways; leading to cell proliferation and cell survival.
References :
1. Birchmeier, C., et al.: Met, metastasis, motility and more. Nat. Rev. Mol. Cell Bio. 2003: 4; 915-925.
2. Fan, S., et al.: The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol. Cell Biol. 2001: 21; 4968-84.
3. Longati, P., et al. Receptor tyrosine kinases as therapeutic targets: the model of the MET oncogene. Curr. Drug Targets. 2001: 2; 41-55.
Product Sheets (By Lot #) :
Research Areas :Cancer, Receptor Tyrosine Kinases