Anti-Phospho-MARK2 (Thr595)(M44-65R)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human MARK2 protein phosphorylated at Threonine 595
Cited Applications :ELISA, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Dog, Bovine, Mouse and Rat
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids surrounding phosphorylated Thr595 of human MARK2 isoform a.
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment.
Sample Data :Western Blot of Anti-Phospho-MARK2 (Thr595) (1:500 diltuion). Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 µg per lane. 
Scientific Background :MARK2 or microtubule affinity-regulating kinase 2 is a member of the Par-1 family of serine/threonine protein kinases. MARK2 is an important regulator of cell polarity in epithelial and neuronal cells and controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins (1). MARK2 is a key target of H. pylori CagA in the disorganization of gastric epithelial architecture underlying mucosal damage, inflammation, and carcinogenesis (2). MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation. MARK2 function in the establishment of T cell polarity following engagement to an APC.
References :
1. Yoshimura, Y. et.al: Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons. . J. Neurosci. 27:13098-13107, 2010.
2. Saadat, I. et.al: Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity. Nature 447: 330-333, 2007.
Product Sheets (By Lot #) :
Research Areas :Invasion/Metastasis, Neurobiology, Ser/Thr Kinases