METTL3/METTL14, Active(M323-380G)

METTL3/METTL14, Active(M323-380G)

  • $259.00


Description :Full-length recombinant human METTL3/METTL14 complex was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_019852

Genbank Number 2 :NM_020961

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Methyltransferase Assay

Molecular Weight :100 kDa (METTL3) and 84 kDa (METTL14)

Gene Aliases :METTL3: IME4; M6A; MT-A70; Spo8
METTL14: None

Scientific Background :Methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) form a stable heterodimer core complex that catalyzes N6-methyladenosine (m6A) RNA methylation in mammalian cells. m6A is an abundant internal modification in messenger RNA and long non-coding RNA. It functions in multiple aspects of developmental regulation, cell cycle progression, cell fate, and the heat shock stress response by affecting aspects of RNA metabolism such as pre-mRNA processing, translation efficiency, transcript stability and miRNA biogenesis.

References :
1. Liu J. et al: A METTL3–METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat. Chem. Biol. 2014; 10: 93–95

2. Wang X. et al: Structural basis of N6-adenosine methylation by the METTL3–METTL14 complex. Nature 2016; 534:575-578

3. Wang P. et al: Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases. Mol. Cell 2016; 63(2):306-317

Product Sheets (By Lot #) :


Research Areas :Neurobiology