Anti-phospho-ERK1/2 (Tyr204)(M29-65BR)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the ERK1/2 protein phosphorylated at tyrosine 204
Cited Applications :WB, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr204
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blots from NIH-3T3 cells using ERK1/2 antibody (lanes 1 and 2) and anti-phospho-ERK1/2 (Tyr204) antibody (lanes 3 and 4).
Sample Data :Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-ERK1/2 (Tyr204) antibody.
Scientific Background :Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is a serine threonine kinase. It plays an integral role of cellular signaling during mitogenesis and differentiation of mitotic cells. ERK is presumed to have a key role in learning and memory (1,2,3). The activity of this kinase is regulated by phosphorylation at Thr202 and Tyr204 (4). Activated ERK1/2 translocates into the nucleus where it phosphorylates various transcription factors (e.g Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta).
References :
1. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
2. Johnson, G L. et al: Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 202 298:1911-1912.
3. Tanoue, T J. et al: Molecular recognitions in the MAP kinase cascades. Cellular Signaling 2003 15:455-462.
4. Ahn, N G.: The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 1993 127-128:201-209.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases