MLK3, Active(M19-11G)
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Description :Recombinant human MLK3 (1-488) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-488
Genbank Number :NM_002419
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~83 kDa
Gene Aliases :MAP3K11, PTK1, SPRK, MLK3, MGC17114, MLK-3
Scientific Background :MLK3 or Mixed-Lineage Kinase 3 is a mitogen-activated protein kinase kinase kinase capable of preferentially activating MAPK8/JNK kinase and functions as a positive regulator of JNK signaling pathway (1). MLK3 can directly phosphorylate and activate IkappaB kinase α and β, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. MLK3 is a signal-integrating kinase with conventional MAP3K catalytic activity and additional noncatalytic functions that contribute to RAF/ERK signalling (2). MLK3 is a component of the BRAF/RAF1 complex and is required for integrity of the complex and for activation of ERK by the complex.
References :
1. Ing, Y. L. et al: MLK-3: identification of a widely-expressed protein kinase bearing an SH3 domain and a leucine zipper-basic region domain. Oncogene 9: 1745-1750, 1994.
2. Chadee, D. N. et al: MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation. Nature Cell Biol. 6: 770-776,2004.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cell Cycle, JNK/SAPK Pathway, Ser/Thr Kinases, JNK/SAPK Pathway, Apoptosis/Autophagy, Cell Cycle, Ser/Thr Kinases