MLK1, Active(M17-11G)

MLK1, Active(M17-11G)

  • $226.00


Description :Recombinant human MLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-433

Genbank Number :NM_033141

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~77 kDa

Gene Aliases :MAP3K9, MEKK9; PRKE1

Scientific Background :MLK1 or Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway. The catalytic domain of MLK1 has amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition, MLK1 contain 2 leu/ile-zipper motifs and a basic sequence near its C-termini (1). MLK1 is threonine (and possibly serine) phosphorylated at multiple sites in the activation loop, with phosphorylation of Thr312 required for full activation (2).

References :
1. Dorow, D S. et al: Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains. Europ. J. Biochem. 213: 701-710, 1993.

2. Durkin, J T. et al: Phosphoregulation of mixed-lineage kinase 1 activity by multiple phosphorylation in the activation loop. Biochemistry. 2004 Dec 28;43(51):16348-55.

Product Sheets (By Lot #) :



Research Areas :Apoptosis/Autophagy, Cell Cycle, JNK/SAPK Pathway, Ser/Thr Kinases, JNK/SAPK Pathway, Apoptosis/Autophagy, Cell Cycle, Ser/Thr Kinases