Anti-phospho-ASK1 (Ser966)(M13-65R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the ASK1 protein phosphorylated at serine 966
Cited Applications :IHC
Cross Reactivity :Human and Mouse
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser966
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-ASK1 (Ser966) antibody.
Scientific Background :ASK1, also known as MAPKKK5, is responsible for activating MKK3, MKK4 (SEK1), and MKK6. ASK1 is activated in cells treated with tumor necrosis factor-alpha and overexpression of ASK1 leads to increased apoptosis (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway leading to the activation of MKK4 and subsequently JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
References :
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases, Cancer, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases