Anti-Phospho-ASK1 (Ser83)(M13-65BR)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the ASK1 protein phosphorylated at Serine 83
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 76-87 on human ASK1 protein.
Purification :Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot of Anti-Phospho-ASK1 (Ser83) against Cos-7 cell lysates after transient infection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 (Ser83 substituted with Ala residue).
Scientific Background :ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
References :
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases