Anti-phospho-MEK5 (Ser311 Ser315)(M06-65R)
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Description :Rabbit Polyclonal Antibody
Species :Rabbit
Tag :
Expression System:
Sequence :
Specificity :Recognizes the MEK1 protein phosphorylated at serine 311/315
Cited Applications :WB
Cross Reactivity :Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Xenopus and Zebrafish
Host :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of rat testes lysate showing specific labeling of the ~49kDa MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen (Peptide) but not by the corresponding dephosphopeptide (not shown).
Scientific Background :MEK5, which is also known as MKK5, is a dual specificity serine/threonine protein kinase that belongs to the MAP kinase kinase family. MEK5 specifically activates ERK5 (1) and MEK5 itself is regulated by MEKK3 (2). Phosphorylation of MEK5 at Ser311 and Thr315 has been implicated in cell proliferation (3). Indeed, there is an important link between MEK5 and metastatic prostate cancer (4).
References :
1. Zhou G et al: Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 1995 270(21):12665-9.
2. Chao T H, et al: MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway. J. Biol. Chem. 1999 274(51):36035-8.
3. Cameron S J. et al: Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation. J. Biol. Chem. 2004 279(2):1506-12.
4. Mehta P B, et al: MEK5 overexpression is associated with metastatic prostate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 2003 22(9):1381-9.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases