Anti-phospho-MEK1 (Thr386)(M02-65CR)
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Description :Rabbit Polyclonal Antibody
Species :Rabbit
Tag :
Expression System:
Sequence :
Specificity :Recognizes the MEK1 protein phosphorylated at threonine 386
Cited Applications :WB
Cross Reactivity :Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate and Xenopus
Host :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of human T47D cells showing specific immunolabeling of the ~45kDa MEK1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: lambda Ptase) The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-MEK1 (Thr386) antibody. The immunolabeling of MEK1 is completely eliminated by lambda Ptase.
Scientific Background :MEK1 is a member of the dual specificity protein kinase family and is also referred to as MAP Kinase Kinase or MKK. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of the MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes including proliferation, differentiation, transcriptional regulation and development (1). MEK1 plays a key role in synaptic plasticity in the brain (2). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK 1 represents a likely target for pharmacological intervention in proliferative diseases such as cancer (3). MAP kinase can phosphorylate MEK1 at Thr292 and Thr386.
References :
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 1995 Jun;9(9):726-35.
2. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
3. Sebolt-Leopold, J S. et al: Blockade of the MAP Kinase Pathway Suppresses growth of colon tumors in vivo. Nature Med. 1999 5: 810-816.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases