• $455.00


Description :Mouse Monoclonal Antibody

Species :Mouse

Specificity :Recognizes the MEK1 protein

Cited Applications :WB, IP

Cross Reactivity :Western blot human MEK1

Immunoprecipitation of human MEK1

MEK1 from other species may also be detectable.

Host / Isotype / Clone# :Mouse, IgG1, 7F1

Immunogen :Recombinant full length humanMEK1 protein

Purification :Affinity purified using Protein G

Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Western Blot :
Representative western blot with Anti-MEK1 (1:1000) using 20ng of MEK1 and MEK2 recombinant protein.

Sample Immunoprecipitation :
Representative immunoprecipitation with Anti-MEK1 (2 µg) using 20ng of MEK1 and MEK2 recombinant protein.

Scientific Background :MEK1 is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).

References :
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995.

2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.

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Research Areas :Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis