Anti-phospho-MARCKS (Ser152 Ser156)(M02-565R)
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Description :Rabbit Polyclonal Antibody
Species :Rabbit
Tag :
Expression System:
Sequence :
Specificity :Recognizes the MARKS protein phosphorylated at serine 152 and serine 156
Cited Applications :WB
Cross Reactivity : Human, Mouse, Rat, Bovine, Chicken, Xenopus and Zebrafish
Host :Rabbit, IgG
Immunogen : corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of rat brain lysate showing specific immunolabeling of the ~87kDa MARCKS protein phosphorylated at Ser152/156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-MARCKS (Ser152 Ser156) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Scientific Background :Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a member of a family of calmodulin binding proteins thatis a target for phosphorylation by protein kinase C (PKC) (1). MARCKS is concentrated in the synapses of neurons where it appears to function in synaptic vesicle cycling. MARCKS has been shown to bind both actin and calmodulin in vitro (2). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (3). Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (4).
References :
1. Ouimet, C C. et al: Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. 1990 J Neurosci 10:1683-1698.
2. Hartwig J H. et al: MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 1993356: 618-622.
3. Stumpo D J. et al: MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 1995 92; 944-948.
4. Verghese G M. et al: Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 1994 269:9361-9367.
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Research Areas :Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Neurobiology, Invasion/Metastasis, Ser/Thr Kinases