LIF(L845-40N)

LIF(L845-40N)

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Description :Recombinant Human LIF was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P15018

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :20.0 kDa

Gene Aliases :LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Recombinant Human Leukemia Inhibitory Factor (LIF)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Sample Cell Assay Data :The ED50 was in found to be <0.05 ng as determined by the dose-dependent proliferation of human erythroleukemic cell line.

Purity :Recombinant Human LIF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3211-86.pdf

Research Areas :Cancer