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Description :Recombinant full-length human LCMT2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_014793
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :90 kDa
Gene Aliases :PPM2; TYW4
Scientific Background :LCMT2, also named tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that involves in the final step of wybutosine biosynthesis pathway (1). Structural study revealed that TYW4 uses one catalytic site for both methylation and methoxycarbonylation process (2).
1. Noma, A., et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 25(10):2142-54, 2006.
2. Suzuki, Y., et al. Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4. Nucleic Acids Res. 37(9):2910-25, 2009.
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Research Areas :Cell Cycle, Metabolic Disorder, Neurobiology