LIMK2, Active(L05-10G)

LIMK2, Active(L05-10G)

  • $226.00


Description :Recombinant full-length human LIMK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :CR456513

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~96 kDa

Gene Aliases :(None)

Scientific Background :LIMK2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain (1). LIM domains are highly conserved cysteine-rich structures that can mediate protein-protein interaction. LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and then LIMK2 in turn phosphorylates cofilin, inhibiting its actin-depolymerizing activity which contributes to Rho-induced reorganization of the actin cytoskeleton (2). LIMK2 is highly expressed in liver, colon, stomach, spleen, brain, kidney, and placenta.

References :
1. Okano, I. Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs. J. Biol. Chem. 270: 31321-31330, 1995.

2. Maekawa, M. Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase. Science 285: 895-898, 1999.

Product Sheets (By Lot #) :


Research Areas :Angiogenesis, Cancer, Cardiovascular Disease, Invasion/Metastasis, Ser/Thr Kinases