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KIF5B-MET (Kex24Mex14), Active (M52-19BG)

KIF5B-MET (Kex24Mex14), Active (M52-19BG)

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Description :Recombinant human fusion KIF5B (1-921a.a., exons 1-24) - MET (963 a.a. - end, exons 14-21) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-921a.a., exons 1-24 (KIF5B) 963 a.a. - end, exons 14-21 (MET)

Genbank Number 1:NM_004521

Genbank Number 2:NM_000245

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :180 kDa

Gene Aliases :KIF5B: KINH, UKHC, KNS
MET: HGFR, RCCP2

Scientific Background :MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kDa (alpha) and 145 kDa (beta). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue (1). Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion (2). The fusion proteins of KIF5B-MET variant has been found in lung adenocarcinomas with the function of oncogenesis (3).

References :
1. Giordano, S. et al: Biosynthesis of the protein encoded by the c-met proto-oncogene. Oncogene. 1989 Nov;4(11):1383-8.

2. Iyer, A. et al: Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene. Cell Growth Differ. 1990 Feb;1(2):87-95.

3. Gow CH, et al: Oncogenic Function of a KIF5B-MET Fusion Variant in Non-Small Cell Lung Cancer. Neoplasia. 2018 Aug;20(8):838-847.

Product Sheets (By Lot #) :

R2854-7.pdf

Research Areas :Cancer