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KIF5B-ALK (Kex24Aex20), Active (A19-19MG)

KIF5B-ALK (Kex24Aex20), Active (A19-19MG)

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Description :Recombinant human fusion KIF5B (1-921a.a., exons1-24) - ALK (1058a.a.-end, exons20-29) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-921a.a., exons1-24 (KIF5B) - 1058a.a.-end, exons20-29 (ALK)

Genbank Number 1:NM_004521

Genbank Number 2:NM_004304

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :195 kDa

Gene Aliases :KIF5B: KINH, UKHC, KNS
ALK: ALK (Ki-1), CD246

Scientific Background :Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. Many chromosomal rearrangements leading to enhanced ALK activity have been described and are implicated in a number of cancer types (1). A fusion protein of kinesin family member 5B (KIF5B) exon 15 to ALK exon 20 has been reported in a primary lung adenocarcinoma (2). Overexpression of KIF5B-ALK increased cell proliferation, migration and invation via activating the STAT3 and PKB (3).

References :
1. Palmer R H, et al: Anaplastic lymphoma kinase: signalling in development and disease. Biochem J. 2009 May 27;420(3):345-61.

2. Takeuchi K, et al: KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. 2009 May 1;15(9):3143-9.

3. Wong DW, et al: A novel KIF5B-ALK variant in nonsmall cell lung cancer. Cancer. 2011 Jun 15;117(12):2709-18.

Product Sheets (By Lot #) :

M2924-7.pdf

Research Areas :Cancer