KAT2B (PCAF), Active (K311-381BG)
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Description : Recombinant human KAT2B (PCAF) (431-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : Sf9 insect cells using baculovirus
Sequence : 431-end
Genbank Number : NM_003884
Specific Activity : Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Acetyltransferase Assay
Molecular Weight : ~70 kDa
Gene Aliases : PCAF, CAF, P/CAF, GCN5L, GCN5L1
Scientific Background : KAT2B (also known as PCAF) is a transcription cofactor with a histone acetyltransferase (HAT) activity. KAT2B protein associates with p300/CBP which is a large nuclear protein that bind to many sequence-specific factors that are involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A (1). KAT2B has histone acetyl transferase activity with core histones and nucleosome core particles indicating that KAT2B plays a direct role in transcriptional regulation (2). PCAF functions as a coordinating factor in the regulation of HIF1A-mediated apoptosis and p53-dependent cell cycle progression.
1. Yang, X.-J. et.al: A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Nature 382: 319-324, 1996.
2. Xu, W. et.al: Mammalian GCN5 and P/CAF acetyltransferases have homologous amino-terminal domains important for recognition of nucleosomal substrates. Molec. Cell. Biol. 18: 5659-5669, 1998.
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Research Areas : Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway,