JAK2 (JH2) (V617F W659A W777A F794H) Protein(J02-16G)

JAK2 (JH2) (V617F W659A W777A F794H) Protein(J02-16G)

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Description :Recombinant human mutant JAK2 JH2 domain (V617F W659A W777A F794H) (536-812) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Species :Human

Tag :GST

Expression System:Sf9 insect cells using baculovirus

Sequence :536-812

Genbank Number :NM_004972

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :JAK2 is a member of intracellular non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway (1). JAK2 has two near-identical phosphate-transferring domains. One domain exhibits the kinase activity while the other stabilizes the JAK conformational structure. JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon (2).

References :1. Lacronique, V. Et al: A TEL-JAK2 fusion protein with constitutive kinase activity in human leukemia. Science, 1997; 278: 1309-1312.
2. Ho, J.M. et al: TEL-JAK2 constitutively activates the extracellular signal-regulated kinase (ERK), stress-activated protein/Jun kinase (SAPK/JNK), and p38 signaling pathways. Blood, 2002; 100: 1438-1448.

Molecular Weight :Observed MW ~56 kDa. Calculated MW ~58 kDa.

Product Sheets (By Lot #) :

U4779-13.pdf

O3913-7.pdf

Research Areas :JAK/STAT Pathway