Immobilized Thrombin, Agarose(T565-31AN)
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Description :Native human thrombin was derived from human plasma.
Matrix: highly cross-linked agarose
Average Particle Size: 90 μm
Ligand Density: 1 mg protein / ml matrix
Maximum Flow Rate, Pressure: 200 cm/h, 1 bar
Sample Data :
Gene Aliases :F2; PT; RPRGL2; THPH; Coagulation Factor II; Factor IIa
Storage, Stability, and Shipping :Store product at 2 - 4oC for up to 6 months.
Applications :In vitro Proteolysis
Digestion Conditions :Catalytic pH Range: 6 ~ 9
Catalytic Temperature Range: 20 ~ 37oC
Catalytic Time Range: 1 ~ 16h
Scientific Background :Human thrombin derived from human plasma is an endolytic serine protease that has a molecular weight of 36 KDa. Thrombin specifically recognizes the consensus sequence Leu-Val-Pro-Arg-▼-Gly-Ser. Thrombin has high proteolytic specificity and hydrolytic efficiency, making it a widely used tool for cleaving purification tag from recombinant fusion proteins. Immobilized human thrombin is covalently cross-linked to an agarose matrix circumventing the need for enzyme removal after cleavage. The resin can be reused 10-20 times after regenerating when proper storage conditions are followed.
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