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IL36G(I896-40CN)

IL36G(I896-40CN)

  • $127.00


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Description :Recombinant Human IL36G was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :Q9NZH8

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :18.9 kDa

Gene Aliases :IL1RP2, IL-1 epsilon, IL1F9, IL1H1

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :Human IL36G is a secreted member of the IL1 family, one of three IL36 genes in humans. The IL1 family members display a conserved 12 b-strand and a b-trefoil configuration, likely due to ascendance from a mutual ancenstral gene. IL36G is expressed primarily in tissues containing epithelial cells, and is both intracellular and secreted. Its primary receptor appears to be a combination of IL-1rp2 and IL-1Racp, the first being primarily expressed on dendritic cells and the second being more widely expressed. Binding of IL36G ligands to this receptor is one of the factors which induces dendritic cell maturation. Its expression is also an agonist for pro-inflammatory cytokines through the NF-kB and Map kinase pathways. Recombinant human IL36G migrates as an 18.9 kDa protein on SDS-PAGE and shares approximately 52% sequence identity with mouse IL36G.

Sample Cell Assay Data :The ED (50) was determined by the dose-dependent proliferation of GieNo3B11 cells and was found to be <0.5ng/mL.

Purity :Recombinant Human IL36G resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3211-74.pdf

Research Areas :