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Description :Recombinant Human IL3 was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P08700

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :15.0 kDa

Gene Aliases :IL-3, Mast cell growth factor, MCGF, Multi-CSF, Hematopoietic growth factor, HCGF, P-cell stimulation factor
Recombinant Human Interleukin-3 (IL3)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Scientific Background :IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL3 shows that glycosylation is not required for activity. IL3 sequences are evolutionarily less well conserved with human and murine IL3 sharing approximately 29% homology (at the protein level) and murine and rat IL3 sharing approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant human IL3 is a non-glycosylated globular protein.

Sample Cell Assay Data :The ED50 as determined by the dose-dependent proliferation of Human TF-1 cells was found less than 0.5 ng/mL

Purity :Recombinant Human IL3 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

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