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Description :Recombinant Human IL11 was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P20809

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :19.0 kDa

Gene Aliases :IL-11, Adipogenesis inhibitory factor, AGIF, INN=Oprelvekin
Recombinant Human Interleukin-11 (IL11)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :IL11 is secreted by bone marrow stromal cells (fibroblasts) and is produced also by a number of mesenchymal cells. The IL11 receptor (alpha chain) utilizes gp130 as its signal transducer, which is also a component of other cytokine receptors. The alpha chain of the IL11 receptor has been identified independently as Etl2. IL-1 promotes primary and secondary immune responses in vitro and in vivo and modulates antigen-specific antibody reactions. IL11 promotes the proliferation of IL6 dependent plasmacytoma cell lines in the presence of neutralizing IL6 antibodies. IL11 also stimulates the T cell dependent development of IgG-secreting B cells in spleen cell cultures. IL11 inhibits the differentiation of pre-adipocytes. It induces the synthesis of some acute phase proteins by hepatocytes.

Sample Cell Assay Data :The ED(50) was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 10^6 units/mg.

Purity :Recombinant Human IL11 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

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