• $675.00


Description :Goat Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the p85 ING5 protein

Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :Human

Host Isotype / Clone# :Goat, IgG

Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 127-140 of human p28 ING5 protein (inhibitor of growth family, member 5)

Purification :Immunoaffinity chromatography

Stability :1yr at –20oC from date of shipment

Sample Data :Western blot analysis is shown using Anti-p28ING5 (1:500 dilution) to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1

Scientific Background :p28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.  Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) encodes the longest isoform

References :
1. Shiseki,M., et al.: p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.  Cancer Res. 2003: 63 (10); 2373-2378.

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Research Areas :Cancer, Cell Cycle