• $675.00


Description :Goat Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the p47ING3 protein

Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator

Cross Reactivity :Human

Host Isotype / Clone# :Goat, IgG

Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 294-304 of Human ING3 protein (Inhibitor of growth family, member 3).

Purification :Immunoaffinity chromatography

Stability :1yr at –20oC from date of shipment

Sample Data :Western blot using Anti-p47ING3 (1:1,000 dilution) shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). Each lane contains approximately 10 µg of RKO whole cell lysate.

Scientific Background :p47ING3 is a tumor suppressor protein similar to ING1 that  can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) that encodes the longest isoform

References :
1. Nagashima,M., et al.: A novel PHD-finger motif protein, p47ING3, modulates p53-mediated transcription, cell cycle control, and apoptosis. Oncogene. 2003: 22 (3); 343-350.

2. Gunduz,M., et al.: Allelic loss and reduced expression of the ING3, a candidate tumor suppressor gene at 7q31, in human head and neck cancers. Oncogene. 2002: 21 (28; 4462-4470.

3. He, G.H., et al.: Phylogenetic Analysis of the ING Family of PHD Finger Proteins. Mol Biol Evol. 2005: 22(1); 104-116.

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Research Areas :Cancer, Cell Cycle