ILK Protein (I06-34G)
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Description :Recombinant full-length human Integrin-linked Kinase (ILK) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number : NM_004517.4
Alternative Name(s) :HEL-S-28; ILK-1; ILK-2; P59; p59ILK
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Integrins are a large-family of cell-extracellular matrix (ECM) and cell-cell adhesion molecules that regulate development and tissue homeostasis by controlling cell migration, survival, proliferation and differentiation. As integrins lack intrinsic enzymatic activity, their signaling crucially depends on recruiting adaptor and signaling proteins. Integrin-linked kinase (ILK) has been identified as a multifunctional player in cell-matrix interactions, cell adhesion, and anchorage-dependent cell growth (1,2). ILK has combined functions as a signal transducer, scaffold protein, and facilitating further protein recruitment within the ILK-PINCH-PARVIN complex (3). ILK is a potential therapeutic target for not only human malignancies, but also diseases such as kidney fibrosis, inflammatory renal diseases, Alzheimer’s disease and cardiovascular diseases (3).
References :1. Hannigan, Gregory E., et al. "Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase." Nature 379.6560 (1996): 91-96.
2. Widmaier, M., Rognoni, E., Radovanac, K., Azimifar, S. B., & Fässler, R. (2012). Integrin-linked kinase at a glance. Journal of cell science, 125(8), 1839-1843.
3. GĂłrska A, Mazur AJ. Integrin-linked kinase (ILK): the known vs. the unknown and perspectives. Cell Mol Life Sci. 2022 Jan 28;79(2):100. doi: 10.1007/s00018-021-04104-1. PMID: 35089438; PMCID: PMC8799556.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Calculated MW ~78 kDa.
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