• $675.00


Description :Rabbit Polyclonal Antibody

Specificity :Recognizes the human IKKε protein phosphorylated at Threonine 501

Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :Human

Host Isotype / Clone# :Rabbit, IgG

Immunogen :The antibody was produced against synthesized peptide corresponding to a region of the protein surrounding phosphorylated Thr501 conjugated to KLH

Purification :Immunoaffinity chromatography and cross adsorption

Stability :1yr at –20oC from date of shipment

Scientific Background :Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity.  IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg.   Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1.  LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKe pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis reasearch.

References :
1. Sweeney SE, et al.: Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3. Arthritis Rheum. 2007: 56(3); 743-752.

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Research Areas :AKT/PKB Pathway, Apoptosis/Autophagy, Inflammation, p38 Pathway