Anti-IKK beta(I03-63R)

Anti-IKK beta(I03-63R)

  • $595.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the IKK beta protein and has no cross response to IKK alpha or IKK gamma

Cited Applications :WB, ELISA, ICC, IF

Cross Reactivity :Human

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta, which differs from corresponding murine sequence by one amino acid.

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody at 1:500 dilution.

Sample Data :Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/ml.

Scientific Background :IKKbeta is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKbeta is stimulated by TNF and IL1 and IKKbeta forms a heterodimer that interacts with NIK (1). Overexpression of catalytically inactive IKKbeta blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKbeta activity in vitro and in vivo by binding to IKKbeta to reduce ATP binding (2).

References :
1. Woronicz, J D. et al: IkappaB kinase-beta: NF-kappa-B activation and complex formation with IkappaB kinase-alpha and NIK. Science 278: 866-869, 1997.

2. Yin, M.-J. et al: The anti-inflammatory agents aspirin and salicylate inhibit the activity of I-kappa-B kinase-beta. Nature 396: 77-80, 1998.

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Research Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy