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Description :Recombinant full-length human HSP70 was expressed in E. coli cells using an N-terminal GST tag.
Tag :GST tag
Sequence :Full Length
Genbank Number :NM_005345
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :ATPase Assay, Western Blot
Molecular Weight :~96 kDa
Gene Aliases :HSPA1A, HSP70-1, HSP72, HSPA1, HSPA1B. DAQB-147D11.1
Scientific Background :HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli.
1. Moran, L A. et al: The major heat-shock protein (hsp70) gene family: related sequences in mouse, Drosophila, and yeast. Can J Biochem Cell Biol. 1983 Jun;61(6):488-99.
2. Pelham, H R: Hsp70 accelerates the recovery of nucleolar morphology after heat shock. EMBO J. 1984 Dec 20;3(13):3095-100.
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Research Areas :Cancer, Inflammation, Cardiovascular Disease, Apoptosis/Autophagy, Cellular Stress, Cancer, p38 Pathway, Cellular Stress