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Description :Recombinant full-length human HSP40 was expressed in E. coli cells using an N-terminal GST tag.
Tag :GST tag
Sequence :Full Length
Genbank Number :NM_006145
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~65 kDa
Gene Aliases :DNAJB1; HSPF1
Scientific Background :HSP40 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP40 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells (1). The intracellular localization of HSP40 is very similar to that of HSP70. Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion) (2).
1. Hattori, H. et al: Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells. Cell Struct. Funct. 1992;17(1):77-86.
2. Kobayashi, Y. et al: Chaperones Hsp70 and Hsp40 suppress aggregate formation and apoptosis in cultured neuronal cells expressing truncated androgen receptor protein with expanded polyglutamine tract. J Biol Chem. 2000 24;275:8772-8
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Research Areas :Cancer, Inflammation, Cellular Stress, Cancer, p38 Pathway, Cellular Stress