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HIPK4, Active(H06-10G)

HIPK4, Active(H06-10G)

  • $226.00


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Description :Recombinant full-length human HIPK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_144685

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~110 kDa

Gene Aliases :FLJ32818

Scientific Background :HIPK4 or homeodomain interacting protein kinase 4 is a serine/threonine protein kinase that can act as a co-repressor of transcription factors (1). HIPK4 has been shown to phosphorylate the human p53 protein at serine 9, both in vitro and in vivo which is important for p53 mediated transcriptional repression. RT-PCR analysis showed moderate expression of HIPK4 in lung and white adipose tissue and weak expression in brain and liver. HIPK4 expression has also been observed in various cancer cell lines (2).

References :
1. Arai, S. et al: Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9. FEBS Lett. 2007;11;581(29):5649-57.

2. Di Stefano, V. et al: Homeodomain-interacting protein kinase-2 activity and p53 phosphorylation are critical events for cisplatin-mediated apoptosis. Exp. Cell. Res. 2004;15;293(2):311-20.

Product Sheets (By Lot #) :

L244-1.pdf

N223-3.pdf

Research Areas :Cancer, Metabolic Disorder, WNT Signaling, Cell Cycle, Ser/Thr Kinases, Cancer, Metabolic Disorder, WNT Signaling, Cell Cycle, Ser/Thr Kinases