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HCC-1 (CCL14)(H811-40N)

HCC-1 (CCL14)(H811-40N)

  • $182.00


Description :Recombinant Human HCC-1 (CCL14) was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Sequence :

Genbank Number :Q16627

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :9.0 kDa

Gene Aliases :C-C motif chemokine 14, Chemokine CC-1, HCC1, HCC-3, HCC-1(1-74), NCC-2, Small-inducible cytokine A14, CCL14, NCC2, SCYA14

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :Hemofiltrate C-C chemokine (HCC)-1 is a recently described monocyte chemoattractant. CCL14 (also known as HCC-1) belongs to the CC chemokine family. Its mature propeptide is a low-affinity agonist of CCR1 that is converted to a high-affinity agonist of CCR1 and CCR5 on proteolytic processing by serine proteases. Determination of the amino acid sequence of HCC-1 revealed four cysteine residues in positions characteristic of the C-C chemokine family, and comparison with the sequences of other chemokines revealed that HCC-1 was most homologous to MIP-1a. However, several functional properties of HCC-1 were atypical of chemokines. Unlike other chemokines, HCC-1 was expressed constitutively in a number of tissues and was present at high concentrations in normal human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes.

Sample Cell Assay Dat :Determined by its ability to chemoattract human monocytes using a concentration range of 2-40 ng/mL.

Purity :Recombinant Human HCC-1 (CCL14) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

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