Anti-HDAC4(H86-63BR)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the HDC4 protein
Cited Applications :ELISA, IF, IC, WB
Ideal working dilutions for each application should be empirically determined by the investigator
Cross Reactivity :Human, Mouse, Rat and Primate
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to the internal region surrounding 600-650 aa of human HDC4 protein
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western Blot of Anti-HDAC4 antibody (1:400). Lane 1: Mouse brain homogenate. Lane 2: Mouse brain homogenate blocked with peptide. Load: 30 µg per lane. 
Scientific Background :HDAC4 or Histone Deacetylase 4 belongs to class II of the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex that represses transcription when tethered to a promoter. HDAC4 does not bind DNA directly, but through transcription factors MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Suppression of HDAC4 binding to NCOR2 and to HDAC3 results in loss of enzymatic activity associated with HDAC4.
References :
1. Wang, A. H. et al: HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Molec. Cell. Biol. 1999: 19; 7816-7827
2. Fischle, W. et al: Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR. Molec. Cell 2002: 9; 45-57
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Research Areas :Cancer, Cell Cycle, Inflammation