Anti-Tyrosine Hydroxylase(H492-363R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the Tyrosine Hydroxylase (TH) protein
Cited Applications :WB, IF, IHC
Cross Reactivity :All mammals
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k Tyrosine Hydroxylase protein.
Scientific Background :Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Tyrosine Hydroxylase antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).
References :
1. Dunkley PR, Bobrovskaya L, et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. 2001.
2. Salvatore MF, Waymire JC, Haycock JW, Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. 2001.
3. Witkovsky P, Gabriel R, Haycock JW, Meller E, Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. 2000.
4. Xu ZQ, Lew JY, Harada K, et al, Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neurosci 82:727-738. 1998.
5. Zhu MY, Klimek V, Haycock JW, Ordway GA, Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. 2000.
6. Zhu MY, Klimek V, Dilley GE, et al, Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. 1999.
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Research Areas :Neurobiology