Anti-phospho-HTT (Ser421)(H390-65R)
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Description :Rabbit Polyclonal Antibody
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Specificity :Recognizes the human Huntington protein phosphorylated at Serine 421
Cited Applications :ELISA, IHC, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 416-424 of human Huntington protein.
Purification :Affinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Immunohistochemical analysis of formalin-fixed and paraffin embedded human brain cerebellum using Anti-Phospho-HTT (Ser421) (1:100 dilution).
Sample Data :Western blot analysis on endogenous protein present in unstimulated human PC-3 whole cell lysate using Anti-Phospho-HTT (Ser421) antibody (1:1,000 dilution).
Scientific Background :Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
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