Anti-phospho-Histone H2A.X (Ser139)(H11-565R)

  • $270.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the Histone H2A.X protein phosphorylated at serine 139

Cited Applications :WB

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X antibody (Lane 1+2) and Anti-phospho-Histone H2A.X (Ser139) (Lane 3+4)

Sample Data :Immunofluorescence staining of methanol-fixing HeLa cells using Anti-phospho-Histone H2A.X (Ser139)

Sample Data :Western blot analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Anti-phospho-Histone H2A.X (Ser139)

References :
1. Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.

2. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.

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Research Areas :Cancer, Cell Cycle, Inflammation, Cancer, Inflammation, Cell Cycle