Anti-phospho-Histone H2A.X (Ser139)(H11-565R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the Histone H2A.X protein phosphorylated at serine 139
Cited Applications :WB
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X antibody (Lane 1+2) and Anti-phospho-Histone H2A.X (Ser139) (Lane 3+4)
Sample Data :Immunofluorescence staining of methanol-fixing HeLa cells using Anti-phospho-Histone H2A.X (Ser139)
Sample Data :Western blot analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Anti-phospho-Histone H2A.X (Ser139)
References :
1. Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
2. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.
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Research Areas :Cancer, Cell Cycle, Inflammation, Cancer, Inflammation, Cell Cycle