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HIPK3, Active(H05-13H)

HIPK3, Active(H05-13H)

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Description :Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :HIS tag

Expression System:Sf9 insect cells using baculovirus

Sequence :163-562

Genbank Number :NM_005734

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~49kDa

Gene Aliases :PKY, YAK1, DYRK6, FIST3

Scientific Background :HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis (1). JNK regulates the expression of HIPK3 in prostate cancer cells and this contributes to increased resistance to Fas receptor-mediated apoptosis by reducing the interaction between FADD and caspase-8 (2). HIPK3 has been reported to phosphorylate FADD and is implicated in multidrug resistance in a number of tumors. HIPK3 increases transcription factor SF-1 activity leading to increased steroidogenic gene expression in response to cAMP signaling.

References :
1. Begley, D.A. et al:Identification and sequence of human PKY, a putative kinase with increased expression in multidrug-resistant cells, with homology to yeast protein kinase Yak1. Gene, 1997; 200 (1-2): 35–43.

2. Curtin, J F. et al: JNK regulates HIPK3 expression and promotes resistance to Fas-mediated apoptosis in DU 145 prostate carcinoma cells. J Biol Chem. 2004 Apr 23;279(17):17090-100.

Product Sheets (By Lot #) :

V178-2.pdf

Research Areas :Cancer, Metabolic Disorder, WNT Signaling, Cell Cycle, Ser/Thr Kinases, Cancer, Metabolic Disorder, WNT Signaling, Cell Cycle, Ser/Thr Kinases