HIPK2, Active(H04-11G)
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Description :Recombinant mouse HIPK2 (153-564) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Mouse
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :153-564
Genbank Number :BC031904
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~72 kDa
Gene Aliases :Stank; 1110014O20Rik; B230339E18Rik
Scientific Background :HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors. HIPK2 enhances expression of p53 target genes that resulting in growth arrest and the enhancement of UV-induced apoptosis that could be inhibited by antisense to HIPK2 (1). HIPK2 as a target for MDM2 mediated ubiquitin-dependent degradation that only occurred in growth-arresting conditions when MDM2 was efficiently induced by p53 (2). The knockdown of HIPK2 using small interfering RNA reduced p53 binding and activation of p53R2 that resulting in impaired UV-induced DNA repair.
References :
1. Hofmann, T. G. et.al: Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2. Nature Cell Biol. 4: 1-10, 2002.
2. Rinaldo, C. et.al: MDM2-regulated degradation of HIPK2 prevents p53Ser46 phosphorylation and DNA damage-induced apoptosis. Molec. Cell 25: 739-750, 2007.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Metabolic Disorder, Ser/Thr Kinases, WNT Signaling, Cancer, Metabolic Disorder, WNT Signaling, Cell Cycle, Ser/Thr Kinases