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GST Protein(G52-30U)

GST Protein(G52-30U)

  • $50.00


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Description :Recombinant artificial sequences GST (1-226) was expressed in E. coli cells and purified on glutathione column.

Species :Artificial

Tag :tag-free

Expression System:E.coli

Sequence :1-226

Genbank Number :U13850

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~25 kDa

Scientific Background :GST or glutathione S-transferase family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are used to create the so-called GST gene fusion system (1). These proteins are capable of multiple reactions with a multitude of substrates. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione (1).

References :
1. Beckett, G.J. and Hayes, J.D. (1987). Glutathione S-transferase measurements and liver disease in man. J. Clin. Biochem. Nutr. 2, 1-24.

Product Sheets (By Lot #) :

F664-2.pdf

Research Areas :Cancer, Neurobiology, Cardiovascular Disease, Cellular Stress, Cancer, Neurobiology, Cardiovascular Disease, Cellular Stress