Due to Xmas holidays, our last days of shipping will be as follow: International Dec 18, 2020. USA: Dec 21, 2020. The shipments will resume as usual on International: Jan 8, 2021 and USA: Jan 4, 2021.
GRK1, Active(R03-10G)

GRK1, Active(R03-10G)

  • $226.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description :Full-length recombinant human GRK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_002929

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~95 kDa

Gene Aliases :GPRK1; RHOK; RK

Scientific Background :GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation (1). Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro (2). It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.

References :
1. Khani, S C. et al: Characterization and chromosomal localization of the gene for human rhodopsin kinase. Genomics 35: 571-576, 1996.

2. Horner, T J. et al: Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities. J Biol Chem. 2005 Aug 5;280(31):28241-50.

Product Sheets (By Lot #) :

E072-3.pdf

B2107-9.pdf

B2143-7.pdf

Research Areas :Angiogenesis, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Metabolic Disorder, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases