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Description :Full-length recombinant human GNAS was expressed in E.coli cells using an N-terminal GST tag.
Tag :GST tag
Sequence :Full length
Genbank Number :NM_000516
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Enzyme Assay
Molecular Weight :70 kDa
Gene Aliases :AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; POH; SCG6; SgVI
Scientific Background :Guanine nucleotide-binding proteins (G proteins) are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits, and function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (GNAS) functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2.
1. Thiele S, et al: Functional characterization of GNAS mutations found in patients with pseudohypoparathyroidism type Ic defines a new subgroup of pseudohypoparathyroidism affecting selectively Gsα-receptor interaction. Hum Mutat. 2011 Jun;32(6):653-60.
2. Pak Y, et al: Direct binding of the beta1 adrenergic receptor to the cyclic AMP-dependent guanine nucleotide exchange factor CNrasGEF leads to Ras activation. Mol Cell Biol. 2002 Nov;22(22):7942-52.
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