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GLK, Active(M25-11G)

GLK, Active(M25-11G)

  • $435.00


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Description :Recombinant human GLK (1-380) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-380

Genbank Number :NM_003618

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~70 kDa

Gene Aliases :MAP4K3; MAPKKKK3; MEKKK3; RAB8IPL1

Scientific Background :GLK is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. GLK is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-α and can lead to the activation of the JNK signaling pathway. GLK can directly activate PKC-θ during TCR signaling and people with systemic lupus erythematosus show considerable enhanced GLK expression and activation of PKC-θ and the kinase IKK in T cells (1). GLK can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM (2).

References :
1. Chuang, H C. et al: The kinase GLK controls autoimmunity and NF-κB signaling by activating the kinase PKC-θ in T cells. Nat Immunol. 2011 Oct 9;12(11):1113-8.

2. Lam, D. et al: MAP4K3 modulates cell death via the post-transcriptional regulation of BH3-only proteins. Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11978-83.

Product Sheets (By Lot #) :

F696-1.pdf

F399-1.pdf

O1025-2.pdf

Z1323-3.pdf

A1291-2.pdf

G1174-1.pdf

 P1591-7.pdf

C2068-4.pdf

B2193-3.pdf

L1885-7.pdf

L1969-10.pdf

P1687-5.pdf

D2410-7.pdf

D2508-8.pdf

R2879-8.pdf

R2736-3.pdf

R2976-8.pdf

Research Areas :JNK/SAPK Pathway, Metabolic Disorder, Ser/Thr Kinases, Metabolic Disorder, JNK/SAPK Pathway, Ser/Thr Kinases