GAPDH Positive Control siRNA (G13-911B)
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Description : GAPDH Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human GAPDH mRNA expression. The synthetic siRNAs (5’-GUG GAG AUU GUU GCC AUC AdTdT-3’ and 5’- UGA UGG CAA CAA UCU CCA CdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Specificity : GAPDH Positive Control siRNA is designed to specifically knock-down human GAPDH expression.
Purity : Single PAGE band
Reconstitution Protocol : Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Format : Lyophilized powder
Spectral Information : Filter: FITC
Excitation max (lambda max): 494 nm
Emission max (lambda max): 520 nm.
Storage and Stability : The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Scientific Background : Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the sixth step of glycolysis – the oxidative phosphorylation of glyceraldehyde-3-phosphate – in the metabolic breakdown of glucose. GAPDH also plays an important role across a diverse array of biological functions such as endocytosis, mRNA regulation, and tRNA export, DNA replication, DNA repair, and apoptosis . GAPDH also serves as an ideal loading control or housekeeping gene .
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Research Areas : Apoptosis/Autophagy, Metabolic Disorder,