Anti-phospho-GSK3 beta (Ser9)(G09-652R)
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Description :Rabbit Polyclonal Antibody
Species :Rabbit
Tag :
Expression System:
Sequence :
Specificity :Recognizes the GSK3 beta protein phosphorylated at serine 9
Cited Applications :WB
Cross Reactivity :Human, Mouse, Rat, Chicken, Bovine, Canine, non-Human Primate, Xenopus and Zebrafish
Host :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of rat cortex lysate showing specific immunolabeling of the ~46kDa GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).
Scientific Background :Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. There have been two isoforms identified, GSK3alpha and GSK3beta. GSK3beta is ubiquitously expressed in human tissues and is implicated in regulating several physiological processes. These include the control of glycogen, protein synthesis by insulin and modulation of the transcription factors AP-1 and CREB. GSK3beta has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (1). GSK3beta is inhibited by phosphorylation at Ser9. Insulin signaling is largely responsible for this mode of GSK3beta inhibition (2).
References :
1. Papkoff, J. et al: WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. 1998 Jun 29;247(3):851-8.
2. Sutherland, C. et al: Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. 1993 Nov 15; 296 (Pt. 1):15-9.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases