GSK3b (C199A), Active(G09-12H)
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Description :Recombinant full-length human GSK3 (C199A) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :Full-length
Genbank Number :NM_001146156
Alternative Name(s) :(None)
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :GSK3 is a serine threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase (1). GSK3 is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3β into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies (2).
References :1. Sutherland C. et al: Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling. Biochem J. 1993 Nov 15;296 ( Pt 1):15-9.
2. Sperber BR, Leight S, Goedert M, Lee VM. Glycogen synthase kinase-3 beta phosphorylates tau protein at multiple sites in intact cells. Neurosci Lett. 1995 Sep 8;197(2):149-53.
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~48 kDa. Calculated MW ~48 kDa.
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