Anti-Phospho-GSKα (Ser21)(G08-65R)
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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human GSK3 alpha protein phosphorylated at Serine 21
Cited Applications :ELISA, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 16-25 of human GSK3 alpha
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment.
Sample Data :Western blot using Affinity Purified Anti-Phospho-GSKα (Ser21) antibody to detect human GSK3A in various human derived 293T cell extracts. Cells were serum starved for 24 h. Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min.
Scientific Background :GSK3α is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and transcription factors (e.g., JUN) (1). GSK3α also plays a role in the WNT and PI3K signaling pathways. Alzheimer disease is associated with increased production and aggregation of amyloid-beta-40 and -42 peptides into plaques. GSK3α is required for maximal production of the beta-amyloid-40 and -42 peptides generated from the amyloid precursor protein by presenilin-dependent gamma-secretase cleavage. In vitro, lithium, a GSK3α inhibitor, blocks the production of the beta-amyloid peptides by interfering with the gamma-secretase step (2).
References :
1. Ali, A. et al: Glycogen synthase kinase-3 : properties, functions, and regulation. Chem. Rev. 101: 2527-2540, 2001.
2. Phiel, C J. et al: GSK-3-alpha regulates production of Alzheimer's disease amyloid-beta peptides. Nature 423: 435-439, 2003.
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Research Areas :WNT Signaling, NfkB Pathway, Neurobiology, Metabolic Disorder, Invasion/Metastasis, Inflammation, Cardiovascular Disease, Cancer, Apoptosis/Autophagy, AKT/PKB Pathway