GRK6, Active(G04-10G)
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Description :Full-length recombinant human GRK6 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :BC017272
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.activity.gif)
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~93 kDa
Gene Aliases :GPRK6; FLJ32135
Scientific Background :GRK6 is a Ser/Thr protein kinase and a member of the G protein-coupled receptor kinase subfamily. GRK6 is present in all tissues with strongest expression in placenta and skeletal muscle. GRK6 can phosphorylate proteins such as rhodopsin, ADRB2 and the leukotriene B4 receptor BLT1 (1). GRK6 phosphorylates G protein-coupled dopamine receptors, thereby regulating their activity and mediating desensitization of the receptors. GRK6 knockout mice showed supersensitivity to the locomotor-stimulating effects of psychostimulants, including cocaine and amphetamine (2).
References :
1. Gaudreau, R. et al: Threonine 308 within a putative casein kinase 2 site of the cytoplasmic tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-protein-coupled receptor-specific kinase 6-mediated desensitization. J. Biol. Chem. 277: 31567-31576, 2002.
2. Gainetdinov, R R. et al: Dopaminergic supersensitivity in G protein-coupled receptor kinase 6-deficient mice. Neuron 38: 291-303, 2003.
Product Sheets (By Lot #) :
Research Areas :Angiogenesis, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Metabolic Disorder, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases