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FGF2(F820-40N)

FGF2(F820-40N)

  • $138.00


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Description :Recombinant Human FGF2 was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P09038

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :17.0 kDa

Gene Aliases  :bFGF, Fibroblast growth factor 2, FGF-2, Heparin-binding growth factor 2, HBGF-2
Recombinant Human Basic Fibroblast Growth Factor (FGF2)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL is recommended. Please note, filter sterilization is a must following reconstitution. This solution can then be diluted into other buffers.

Scientific Background :Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

Sample Cell Assay Data :The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.

Purity :Recombinant Human FGF2 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.

Product Sheets (By Lot #) :

G3211-34.pdf

Research Areas :Cancer